p iκb Search Results


phosphorylated p iκb α  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology phosphorylated p iκb α
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Phosphorylated P Iκb α, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb/p-I%CE%BAB-%CE%B1+Antibody/pm26865401-30-8-33
Average 96 stars, based on 1 article reviews
phosphorylated p iκb α - by Bioz Stars, 2026-09
96/100 stars
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rabbit anti-p-iκb yp0151  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company rabbit anti-p-iκb yp0151
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Rabbit Anti P Iκb Yp0151, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rabbit anti-p-iκb yp0151 - by Bioz Stars, 2026-09
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iκb-α  (Bioworld Antibodies)
90
Bioworld Antibodies iκb-α
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Iκb α, supplied by Bioworld Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb/p+i%CE%BAb++s32+s36+antibody/pmc06657963-69-39-41
Average 90 stars, based on 1 article reviews
iκb-α - by Bioz Stars, 2026-09
90/100 stars
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p-iκb mbs2600002  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology p-iκb mbs2600002
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
P Iκb Mbs2600002, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb/p+i%CE%BAb+mbs2600002/pmc10420321-191-14-16
Average 90 stars, based on 1 article reviews
p-iκb mbs2600002 - by Bioz Stars, 2026-09
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p-iκb (ser36) (cat. no. gt1298) antibody  (GeneTex)
90
GeneTex p-iκb (ser36) (cat. no. gt1298) antibody
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
P Iκb (Ser36) (Cat. No. Gt1298) Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb/p+I%CE%BAB++Ser36+++Cat++No++GT1298+Antibody/pm39034914-45-11-20
Average 90 stars, based on 1 article reviews
p-iκb (ser36) (cat. no. gt1298) antibody - by Bioz Stars, 2026-09
90/100 stars
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p-iκb  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company p-iκb
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
P Iκb, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb/p+i%CE%BAb+antibody/pm33045564-70-45-47
Average 90 stars, based on 1 article reviews
p-iκb - by Bioz Stars, 2026-09
90/100 stars
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p-iκb kinase α antibody  (Baiao Pharmaceuticals)
90
Baiao Pharmaceuticals p-iκb kinase α antibody
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
P Iκb Kinase α Antibody, supplied by Baiao Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb/p+i%CE%BAb+kinase+%CE%B1+antibody/pmc08026846-49-0-6
Average 90 stars, based on 1 article reviews
p-iκb kinase α antibody - by Bioz Stars, 2026-09
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nfkbia(ser32/36) polyclonal antibody  (Bioss)
94
Bioss nfkbia(ser32/36) polyclonal antibody
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Nfkbia(ser32/36) Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb/NFKBIA(Ser32%2F36)+Polyclonal+Antibody/custom%40bs-2513r%4035625074
Average 94 stars, based on 1 article reviews
nfkbia(ser32/36) polyclonal antibody - by Bioz Stars, 2026-09
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nfkbia(tyr305) polyclonal antibody  (Bioss)
92
Bioss nfkbia(tyr305) polyclonal antibody
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Nfkbia(tyr305) Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb/NFKBIA(Tyr305)+Polyclonal+Antibody/bioss___bs-5515r
Average 92 stars, based on 1 article reviews
nfkbia(tyr305) polyclonal antibody - by Bioz Stars, 2026-09
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rabbit polyclonal antibodies against phosphorylated iκb-α (p-iκb-α  (ImmunoWay Biotechnology Company)
90
ImmunoWay Biotechnology Company rabbit polyclonal antibodies against phosphorylated iκb-α (p-iκb-α
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Rabbit Polyclonal Antibodies Against Phosphorylated Iκb α (P Iκb α, supplied by ImmunoWay Biotechnology Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rabbit polyclonal antibodies against phosphorylated iκb-α (p-iκb-α - by Bioz Stars, 2026-09
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p iκb  (Affinity Biosciences)
86
Affinity Biosciences p iκb
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
P Iκb, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb/anti+i%CE%BAb+p/pm40784326-52-9-13
Average 86 stars, based on 1 article reviews
p iκb - by Bioz Stars, 2026-09
86/100 stars
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anti p iκb antibody  (Wanleibio)
86
Wanleibio anti p iκb antibody
Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of <t>IκB-α</t> was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.
Anti P Iκb Antibody, supplied by Wanleibio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+i%CE%BAb/anti+antibody+i%CE%BAb+p/pm35797025-90-12-20
Average 86 stars, based on 1 article reviews
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Image Search Results


Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of IκB-α was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.

Journal: International journal of molecular medicine

Article Title: Ginsenoside Rg1 exerts a protective effect against Aβ₂₅₋₃₅-induced toxicity in primary cultured rat cortical neurons through the NF-κB/NO pathway.

doi: 10.3892/ijmm.2016.2485

Figure Lengend Snippet: Figure 2. Ginsenoside Rg1 (Rg1) attenuates amyloid β-peptide 25-35 (Aβ25-35)-induced nuclear factor κ-light-chain‑enhancer of activated B cells (NF-κB) activation. Primary cultured neurons were preincubated with 20 µM Rg1 for 24 h, and subsequently treated with 10 µM Aβ25-35 for another 24 h. (A) The fluo rescence images indicated NF-κB translocation to the nucleus after Aβ25-35 treatment (as shown by arrows). Scale bar, 50 µM. (B and C) After stimulation with Aβ25-35 and 150 µM hydrogen peroxide (H2O2) solution for 24 h, the nucleoprotein was extracted using a nuclear and cytoplasmic protein extraction kit. NF-κB expression was detected by western blot analysis. Lamin B was used as a housekeeping protein for the nuclear protein and GAPDH was used for cytoplasmic protein. (D) Phosphorylation of IκB-α was detected by western blot analysis. Data are represented as the means ± standard deviation of three independent experiments. The statistical significance was defined using Student's t-test as *P<0.05, **P<0.01, ***P<0.001 vs. control group, #P<0.05, ##P<0.01 vs. model group. N, nuclear fractions; C, cytoplasmic fractions.

Article Snippet: Primary antibodies against NF-κB (p65) (sc-372), IκB-α (sc-371), phosphorylated (p-)IκB-α (sc-8404), lamin B (sc-6217), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (sc-47724), inducible nitric oxide synthase (iNOS) (sc-651), Bcl-2 (sc-7382), Bax (sc-7480) were all purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA).

Techniques: Activation Assay, Cell Culture, Translocation Assay, Protein Extraction, Expressing, Western Blot, Phospho-proteomics, Standard Deviation, Control